Len's Subjects

Len's Subjects were a group of people who were previously part of Len's experiment on humanity. Although they were not all humans, Yoshi being one of many examples, they were all crucial to the experiment. They do, however, all share one thing in common: Len gave them all a piece of jewelry or an otherwise precious object. With these objects, they would be "reiterated" as long as they collected a 1-Up Mushroom, 100 coins, or otherwise gained an extra life. They all started out with three lives.

Many things can change from iteration to iteration, only the spirits of the subjects are ensured to reiterate, although it is very common for reiterations to be carbon copies of the last. Even so, details such as personalities, species, race, and genetic makeups can be completely changed from iteration to iteration. Some examples of past iterations lingering in the current iterations' minds do exist, such as Mario and Marchionne. Some subjects have the power to save and load.

Riba/Abir
Riba is the first known subject, and may very well be the first subject period. In his Abir iteration, he accepted one of Len's treasures. He traded his soul for an engagement ring so he could propose to Edith, with no monetary cost. When Abir died, be it to an assassin in the unaltered timeline, or Allen in the altered timeline, Len made him a demon. We actually get a glimpse into the reiteration process in this scene. If you are to be reiterated, directly after your death, you are plunged into a sort of large concentration of liquid. But you can breathe it like air unless Len decides otherwise. Then, you are reborn.

Riba died many times after this, until he was freed of the curse of rebirth in the ending of Super Mario Continuance (REITERATED).

Mario/Marchionne
One day, Marchionne Evangelisti was taking a walk in the Silent Forest, when he was approached by Len. She told him of his impending fate to be executed. At first, he didn't believe her. But when she told him of the sin he had committed, a sin which nobody even knew of besides himself and a few others, he believed her. She then handed him a brooch. It could grant him one wish. Len then disappeared upon Marchionne asking her if she was God.

When he was imprisoned, he wished upon the brooch to be reborn with Luciano and Aurora, in a happier life. This wish was granted, and he was reborn as Mario. Luciano was reborn as Luigi. And Aurora was reborn as Princess Peach.

Mario, Luigi and Peach died many times in their dangerous lives. But every time, they were brought back by Len or, in one case, the outside influence of Rosalina in a new iteration. They were very happy, just as Marchionne had wished. This was all until they all entered Aduraice Mansion. Here, they died many times until Mario was able to break the seal by not saving at all in one iteration. When he eventually died, he was reborn back in the Mushroom Kingdom. A super re-iteration had occurred, and everybody who had died in the Corpse Party had been brought back. Even those who weren't Len's subjects such as Bowser and the Aduraices. There has never been another iteration since.

Princess Peach/Misery
Aurora, real name Misery, was a witch who was brought into Evangeline under the guise of her being Marchionne's maid. When he was executed, she poisoned herself. However, she possessed one of Len's artifacts. She wished upon the artifact to be reborn into a happier life with Marchionne. Centuries later, she was reborn as Princess Peach of the Mushroom Kingdom, with Mario, Marchionne's reiteration, as her knight.

Interestingly, Peach has Marchionne's brooch on the chest of her dress. This is because a Toad had found Marchionne's brooch in a forest just outside of Evangeline and decided to keep it. When news came about that a new princess was about to be born, the Toad that had found the brooch decided to donate it to the royal Toadstool family that Peach was going to be born into. The moon and star symbol on the brooch have faded with time, however.

Prince Peasley/Paisley
Paisley was a young woman who was the unmarried love interest of Luciano Evangelisti. However, she died while birthing his child. At some point in her life, she accepted a brooch from Len. She was then reiterated many centuries later as a Beanish. In fact, this Beanish was the prince of the BeanBean Kingdom. And his name was Prince Peasley. This is one of the rare documented examples in history of a subject being reborn as both a different gender and species. Interestingly, Luciano's reiteration, Luigi Mario, showed romantic feelings towards Prince Peasley.